Stimulated human leukocytes cause activating mutations in the K-ras proto-oncogene
β Scribed by Jackson, Janis Harline; Vollenweider, Maya; Hill, Jennifer; Rodriguez, Henry; Schwabacher, Alan W; Mitra, Gopa; Kuo, Chao-Yu
- Book ID
- 110060732
- Publisher
- Nature Publishing Group
- Year
- 1997
- Tongue
- English
- Weight
- 350 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0950-9232
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## Abstract The mutagenicity of oxygen free radicals was studied in a forward mutation system. pEC plasmid containing the human cβHaβ__ras__β1 protoβoncogene was reacted with oxygen free radicals generated by Cu^2+^ and H~2~O~2~ and was then transfected into NIH/3T3 cells. Transformed foci were obs
DNA isolated from blood or bone-marrow samples from 18 patients with acute non-lymphocytic leukemia (ANLL) and 14 patients with acute lymphocytic leukemia (ALL) was analyzed for the presence of mutations in the N-ras gene. Using synthetic oligonucleotide probes we detected mutations in 5 cases of AN
Detection of K