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Mutation analysis of the N-ras proto-oncogene in active and remission phase of human acute leukemias

✍ Scribed by H.-P. Senn; Ch. Trǎn-Thang; A. Wodnar-Filipowicz; J. Jiricny; M. Fopp; A. Gratwohl; E. Signer; W. Weber; Ch. Moroni

Publisher
John Wiley and Sons
Year
1988
Tongue
French
Weight
634 KB
Volume
41
Category
Article
ISSN
0020-7136

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✦ Synopsis

DNA isolated from blood or bone-marrow samples from 18 patients with acute non-lymphocytic leukemia (ANLL) and 14 patients with acute lymphocytic leukemia (ALL) was analyzed for the presence of mutations in the N-ras gene. Using synthetic oligonucleotide probes we detected mutations in 5 cases of ANLL; 4 GGT + GAT transitions in codon I2 and one CAA + AAA transversion in codon 61. One case exhibited homozygosity for the mutation. No mutations could be detected at these codons in the DNA of the 14 ALL patients. In a followup study with 3 of the above 5 patients, the mutation could no longer be detected in 2 cases following successful induction of clinical remission by chemotherapy. However, the mutated N-ras persisted in one patient who did not achieve remission. We show that oligonucleotide hybridization is a sensitive assay for the detection of N-ras point mutations, which in ANLL could be used to follow the fate of the leukemic clone during (and after) therapy. * * 1201-c * * 12p2-A * * 12~2-T * * 12p2-c * * 13111-A GTT GGA 13111-T

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