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Steroid hormone specifically binds to rat kidney plasma membrane

✍ Scribed by Iñaki Ibarrola; Kepa Ogiza; Aida Marino; José M. Macarulla; Miguel Trueba

Publisher
Springer US
Year
1991
Tongue
English
Weight
424 KB
Volume
23
Category
Article
ISSN
0145-479X

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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5