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Characteristics of steroid hormone binding systems in hepatocyte plasma membranes

✍ Scribed by Yu. P. Denisov; P. V. Sergeev; N. P. Saksonov; G. V. Shutko


Publisher
Springer US
Year
1980
Tongue
English
Weight
365 KB
Volume
89
Category
Article
ISSN
0007-4888

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Characterization by photoaffinity labeli
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The mechanism of steroid uptake by the cell remains controversial. [3H]R5020 was utilized to characterize by photoaffinity labeling the steroid binding site in plasma membrane. This binding was saturable, reversible and had one type of binding site (Kd = 33 +/- 4 nM, Bmax = 32 +/- 2 pmol/mg). [3H]R5