Stereotaxic injection of GD1a ganglioside induces limited recovery of striatal dopaminergic system in MPTP-treated aging mice

✍ Scribed by M.D I. Date; M. F. D. Notter; S. Y. Felten; D. L. Felten

• Book ID: 102911975
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 656 KB
• Volume: 28
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.490280409

✦ Synopsis

The systemic administration of 1-methyl-4-phenyl-1,2,3,64etrahydropyridine (MPTP) to young (2 months old) and aging (12 months old) C57BL/6 mice (4 X 20 mg/kg i.p. given 12 hr apart) reduced tyrosine hydroxylase (TH)-immunoreactive (IR) fibers in the striatum and reduced dopamine (DA) concentration to 35% of controls in young and 22% of controls in aging mouse brain 5 weeks after administration. Stereotaxic injection of GDla ganglioside (3 X 100 pg, 5 days apart) into the striatum of MPTPtreated young mice restored striatal DA concentration to 52% of the control concentration 5 weeks after MPTP injection. Similar injections of GDla ganglioside restored striatal DA concentration of MPTPtreated aging mice to only 31% of the control concentration. Immunocytochemical analysis showed significant recovery of TH-IR fibers in the striatum of MPTP-depleted young mice treated with GDla ganglioside, while TH-IR fibers in the striatum of MPTPdepleted aging mice treated with GDla ganglioside showed less recovery. We conclude that treatment of MPTP-depleted aging mice with GDla ganglioside results in more limited recovery in the nigrostriatal DA system than in young mice.