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Stereochemical outcome of the hydrolysis reaction catalyzed by the EcoRV restriction endonuclease

✍ Scribed by Grasby, Jane A.; Connolly, Bernard A.


Book ID
120401094
Publisher
American Chemical Society
Year
1992
Tongue
English
Weight
873 KB
Volume
31
Category
Article
ISSN
0006-2960

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When E. coli cells carrying the plasmid pLG13 (coding for the newly discovered type II restriction endonuclease EcoRV) are infected with phage T3 or T7, only T7 is able to replicate normally. T3 wild-type as well as its ocr- mutants are subject to DNA restriction in vivo and in vitro. The EcoRV enzy