Stereochemical Features of C-methylations on the Path to Δ24(28)-Methylene and Δ24(28)-Ethylidene Sterols: Studies on the Recombinant Phytosterol Methyl Transferase from Arabidopsis thaliana

✍ Scribed by Tong Yusen; Brian S McCourt; De-an Guo; Anil T Mangla; Zhou Wen-Xu; Mark D Jenkins; Zhou Wen; Monica Lopez; W.David Nes

• Book ID: 104257734
• Publisher: Elsevier Science
• Year: 1997
• Tongue: French
• Weight: 533 KB
• Volume: 38
• Category: Article
• ISSN: 0040-4039
• DOI: 10.1016/s0040-4039(97)01386-5

✦ Synopsis

Using a homogenate prepared from Escherichiacoli cells that express the sterol methyl transferase (SMT) gene of Arabidqmis thaliana,migration of the hydrogen atom at C-24 to C-25 from the Re-face of the double bond was demonstmted in the biosynthesis of [27-13C] 24(28)-methylenezymosterol (fecosterol) from [27-13c]zymosterol and the chirality of the C-25 stereoeenter (25R) was found to be retained after the stereospeeific conversion of [27-13C]24@)methylenezymosterol to 27-13C -ethylidenecholest-8-en-3~-ol. @ 1997ElsevierScienceLtd.