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Overexpression, Purification, and Stereochemical Studies of the Recombinant (S)-Adenosyl-l-methionine: Δ24(25)- to Δ24(28)-Sterol Methyl Transferase Enzyme fromSaccharomyces cerevisiae

✍ Scribed by W.David Nes; Brian S. McCourt; Wen-xu Zhou; Jianzhong Ma; Julie A. Marshall; Lauri-Ann Peek; Michael Brennan


Book ID
115567125
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
250 KB
Volume
353
Category
Article
ISSN
0003-9861

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Using a homogenate prepared from Escherichiacoli cells that express the sterol methyl transferase (SMT) gene of Arabidqmis thaliana,migration of the hydrogen atom at C-24 to C-25 from the Re-face of the double bond was demonstmted in the biosynthesis of [27-13C] 24(28)-methylenezymosterol (fecostero