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Stereochemical Course of Escherichia coli RNase H.

✍ Scribed by Agnieszka Krakowiak; Alina Owczarek; Maria Koziolkiewicz; Wojciech J. Stec


Book ID
101936916
Publisher
John Wiley and Sons
Year
2003
Weight
48 KB
Volume
34
Category
Article
ISSN
0931-7597

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Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-

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✍ Horiuchi, Takashi ;Maki, Hisaji ;Sekiguchi, Mutsuo πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 632 KB

Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed