Stereochemical Course of Escherichia coli RNase H.
β Scribed by Agnieszka Krakowiak; Alina Owczarek; Maria Koziolkiewicz; Wojciech J. Stec
- Book ID
- 101936916
- Publisher
- John Wiley and Sons
- Year
- 2003
- Weight
- 48 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0931-7597
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Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-
Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed