Stereochemical Course of Escherichia coli RNase H
✍ Scribed by Agnieszka Krakowiak; Alina Owczarek; Maria Koziołkiewicz; Wojciech J. Stec
- Book ID
- 101355627
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 252 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1439-4227
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Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-
Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed