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Stepwise gradient development in thin-layer chromatography : IV. Miniaturized generators for continuous and stepwise gradients

✍ Scribed by G. Matysik; E. Soczewiński

Publisher: Elsevier Science
Year: 1988
Tongue: English
Weight: 609 KB
Volume: 446
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)94443-1

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✦ Synopsis

A miniaturized generator of continuous gradients for equilibrium sandwich chambers with a glass distributor is described. The gradient profile is controlled by the initial concentration of the weaker eluent and its initial volume and by the composition and rate of delivery of the stronger eluent. Stepwise gradients are generated by delivery of eluents of increasing concentration of the stronger solvent from reservoirs in accordance with the gradient programme. Comparison of densitograms of thin-layer chromatograms from isocratic and gradient elution (continuous or stepwise) showed the considerable improvement in separation under gradient elution conditions.

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