Gradient elution in liquid chromatography : IV. Verification of the theoretical relationships for elution characteristics (retention volume, band width, resolution, plate number) in adsorption chromatography on silica using stepwise and combined (stepwise—gradient) elution and some considerations concerning solvent-programmed and isocratic elution chromatography
✍ Scribed by Pavel Jandera; Jaroslav Churáček
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 914 KB
- Volume
- 104
- Category
- Article
- ISSN
- 1873-3778
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✦ Synopsis
The verification of the theoretical equations for the elution characteristics in stepwise and combined two-step isocratic-gradient elution chromatography is described for four N,N-dimethyl-p-aminobenzeneazobenzoyl amides chromatographed on Porasil A using a binary mobile phase consisting of ethyl acetate and cyclohexane. Good agreement between the experimental and calculated values was found. The resolutions in isocratic, simple gradient, stepwise and combined two-step isocraticgradient elution are compared and the results achieved in the gradient elution chromatography of some N,N-dimethyl-p-aminobenzeneazobenzoyl amides and esters on silica are presented.