The effects of parathyroid hormone (PTH) on 1,4,5-inositol triphosphate (1,4,5-IP 3 ) and intracellular free calcium (Ca i 21 ) in osteoblasts are variable, whereas adenylate cyclase activity is consistently stimulated. Cyclic AMP is considered a mediator in the contractile effects of PTH on osteobl
Staurosporine enhances parathyroid hormone-induced calcium signal in UMR-106 osteoblastic cells
β Scribed by Suk Kyeong Lee; Paula H. Stern
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 428 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0253-6269
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We used the osteogenic sarcoma cell line, UMR-106-01, to determine whether the rise in free cytosolic Ca2+ concentration ([Ca"],) and cellular cAMP following PTH stimulation are able to be regulated independently. For this purpose, we compared the effect of a PTH antagonist, stimulation of protein k
## Abstract 1,25βDihydroxyvitamin D~3~ (1,25(OH)~2~D~3~) treatment of osteoblastic cells was shown previously to attenuate Parathyroid hormone (PTH) response by inhibiting adenylyl cyclase (AC) activity. In this study, we have investigated the mechanism by which 1,25(OH)~2~D~3~ inhibits AC in rat o