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Dissociation between parathyroid hormone-stimulated cAMP and calcium increase in UMR-106-01 cells

✍ Scribed by Bradley S. Merritt; Dean T. Yamaguchi; Jacob Green; Charles R. Kleeman; Shmuel Muallem

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
987 KB
Volume
152
Category
Article
ISSN
0021-9541

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✦ Synopsis

We used the osteogenic sarcoma cell line, UMR-106-01, to determine whether the rise in free cytosolic Ca2+ concentration ([Ca"],) and cellular cAMP following PTH stimulation are able to be regulated independently. For this purpose, we compared the effect of a PTH antagonist, stimulation of protein kinase C, augrnentation by prostaglandins, and the time course of desensitization of the two cellular responses. Two X M of the PTH antagonist '*"Nle '"Tyr-bPTH(3-34) arnide ([Nle,Tyr]hPTH(3-34)A) wa5 reqtiired to inhibit lopL' M bPTH(1-34)-stimulatcd cAMP generation by 50%. 1 0-7 M bPTH(1-34) completely overcame the inhibition induced by loph M 1Nle;ryr]bPTH(3-34)A. Only 7 x lo-" M and 2.7 x l o p 7 M [Nle,Tyr]bPTH(3-34)A were required to half maximally inhibit the D 1992 WILEY-LTSS, INC

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