State and specific growth estimation in heterologous protein production by Pichia pastoris
✍ Scribed by José M. Barrigón; Ramon Ramon; Isabel Rocha; Francisco Valero; Eugénio C. Ferreira; José L. Montesinos
- Book ID
- 111994648
- Publisher
- American Institute of Chemical Engineers
- Year
- 2011
- Tongue
- English
- Weight
- 983 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0001-1541
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The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of recombinant proteins has become increasing popular in recent times. P. pastoris is easier to genetically manipulate and culture than mammalian cells and can be grown to high cell densities. Equally importa
Continuous fermentation was applied to the production of recombinant human chymotrypsinogen B (hCTRB) by the methylotrophic yeast Pichia pastoris as a tool for the kinetic analysis of growth and product formation. Using methanol as the sole source of carbon, energy, and induction, cell growth could