Staining of proteins after SDS-polyacrylamide gel electrophoresis by ethidium bromide
โ Scribed by Igor V. Shevelev; Alexander N. Tretyakov; Oleg K. Kaboev
- Book ID
- 114392973
- Publisher
- Elsevier
- Year
- 1996
- Weight
- 82 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1366-2120
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๐ SIMILAR VOLUMES
A rapid and simple method for isoelectric focusing of proteins separated by SDS-polyacrylamide gel electrophoresis was described. The focusing system was composed of a polyacrylamide gel layer (7 cm in height) and a solution layer (1.2 cm in height) superimposed on the gel layer. Both layers contain
Insulin, hemoglobin, egg albumin, gamma globulin, bovine serum albumin, and phosphorylase a were separated by SDS-polyacrylamide gel electrophoresis, and continuously recovered for quantitation in aqueous solution using a new technique. The proteins, which were eluted in the order of their molecular