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Staining method of poly(3-hydroxyalkanoic acids) producing bacteria by nile blue

โœ Scribed by Shiro Kitamura; Yoshihara Doi


Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
355 KB
Volume
8
Category
Article
ISSN
0951-208X

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โœ William J. Page; Chistopher J. Tenove ๐Ÿ“‚ Article ๐Ÿ“… 1996 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 401 KB

Fluorescence from poly-P-hydroxybutyrate (PHB) inclusions inside Azotobacter vinelandii UWD cells stained with Nile blue A was shown to be proportional to PHB concentration. The intensity of the fluorescence was greatest in native, fluid inclusions and was the least in extracted, crystallized granul