Quantitation of poly-β-hydroxybutyrate by fluorescence of bacteria and granules stained with Nile blue A

✍ Scribed by William J. Page; Chistopher J. Tenove

• Publisher: Springer-Verlag
• Year: 1996
• Tongue: English
• Weight: 401 KB
• Volume: 10
• Category: Article
• ISSN: 0951-208X
• DOI: 10.1007/bf00184017

✦ Synopsis

Fluorescence from poly-P-hydroxybutyrate (PHB) inclusions inside Azotobacter vinelandii UWD cells stained with Nile blue A was shown to be proportional to PHB concentration. The intensity of the fluorescence was greatest in native, fluid inclusions and was the least in extracted, crystallized granules. However, isolated air-dried PHB granules also were proportionally stained with Nile blue A. The results show that Nile blue A can be used in the quantitative determination of PHB in a variety of cells.