A staining procedure for lipoxygenase in electrophoretic gels based on the oxidation of a dye, 3,3'-dimethoxybenzidine hydrochloride, by hydroperoxides is described. The specificity of the stain to the dye was established by enzyme inhibition, by use of nonsubstrates, by enzyme heat-inactivation and
Staining for soybean lipoxygenase activity in electrophoretic gels
β Scribed by Heinisch, O. ;Kowalski, E. ;Tauscher, B. ;Ludwig, H.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Weight
- 160 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0931-5985
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β¦ Synopsis
A staining method for lipoxygenase in electrophoretic gels is described which is based on the staining of hydroperoxides, as reaction products of added substrates, by N,N-dimethyl-p-phenylenediamine.
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