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Stable isotope labelling and quantitative determination of enalaprilate in human plasma by GC–NICI–MS

✍ Scribed by H. J. Leis; H. Gleispach; W. Windischhofer

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
423 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

A method for the quantitative determination of enalaprilate, the active metabolite of the angiotensin‐converting enzyme inhibitor enalapril, is presented. The asay is based on gas chromatography–negative ion chemical ionization–mass spectrometry. The preparation of ^18^O~4~‐labelled enalaprilate for use as an internal standard is described. The method involves solid phase extraction and derivation to the pentafluorobenzyl diester–trifluoroacetamide derivatives. The detection limit was found to be 0.5 pg, and a lower limit of quantitation was reached down to 40 pg ml^−1^ plasma. The method was applied to pharmacokinetics of the metabolite in humans.

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