Stable Expression of Rat Cytochrome P-450IIB1 cDNA in Chinese Hamster Cells (V79) and Metabolic Activation of Aflatoxin B1
β Scribed by Johannes Doehmer, Satish Dogra, Thomas Friedberg, Solange Monier, Milton Adesnik, Hansruedi Glatt and Franz Oesch
- Book ID
- 123635897
- Publisher
- National Academy of Sciences
- Year
- 1988
- Tongue
- English
- Weight
- 938 KB
- Volume
- 85
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/32240
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Chinese hamster V79 fibroblasts, frequently used as target cells in short-term tests for mutagenicity, do not possess measurable monooxygenase activity; in particular, enzymatic oxidation of testosterone (T) cannot be demonstrated. If these V79 cells, however, had been transfected with the cDNAencod
In continuation of our work toward the establishment of a working cell bank for metabolic and toxicological studies, V79 Chinese hamster cells were genetically engineered for stable expression of rat cytochrome P450IA2. Full-length cDNA encoding rat P450IA2 was obtained by searching a cDNA library m