Stable expression of fluorescently tagged proteins for studies of mitosis in mammalian cells
β Scribed by Wadsworth, Patricia; Rusan, Nasser M; Tulu, U Serdar; Fagerstrom, Carey
- Book ID
- 109937760
- Publisher
- Nature Publishing Group
- Year
- 2005
- Tongue
- English
- Weight
- 332 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1552-4450
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FIG. 3. Comparison of the rapid S1 nuclease mapping method with the classical ribonuclease protection technique. Ten micrograms of Biochemical and functional analysis of proteins gentotal RNA from MCF7 was hybridized either with the labeled singleerally requires their detection and purification from
A cell's biochemistry is now known to be the biochemistry of molecular machines, that is, protein complexes that are assembled and dismantled in particular locations within the cell as needed. One important element in our understanding has been the ability to begin to see where proteins are in cells