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Vectors for Expression of Protein-A-Tagged Proteins in Vertebrate Cells

✍ Scribed by Peter Uetz; Rolf Zeller

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
116 KB
Volume
237
Category
Article
ISSN
0003-2697

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✦ Synopsis

FIG. 3. Comparison of the rapid S1 nuclease mapping method with the classical ribonuclease protection technique. Ten micrograms of Biochemical and functional analysis of proteins gentotal RNA from MCF7 was hybridized either with the labeled singleerally requires their detection and purification from stranded DNA probe (lane 2) or a labeled riboprobe mapping the cultured cells transfected by appropriate expression same region (lane 1) and then digested with the appropriated enzyme.

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## Abstract An expression vector was specifically designed for use in Chinese hamster ovary (CHO) cells to enhance the level of protein production in a transient expression system. Two key components that can increase protein production transiently are the promoter used to drive recombinant gene ex