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Stabilizing and suicide-peroxide protecting effect of Ni2+on horseradish peroxidase

✍ Scribed by K. Nazari; A. Mahmoudi; R. Khodafarin; A. A. Moosavi-Movahedi; A. Mohebi


Book ID
113009339
Publisher
Iranian Chemical Society
Year
2005
Tongue
English
Weight
119 KB
Volume
2
Category
Article
ISSN
1735-207X

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## Abstract Phthalic anhydride (PA) modification stabilizes horseradish peroxidase (HRP) by reversal of the positive charge on two of HRP's six lysine residues. Native and PA‐HRP had half‐inactivation temperatures of 51 and 65Β°C and half‐lives at 65Β°C of 4 and 17 min, respectively. PA‐HRP was more