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Stabilization of a binary protein complex by intein-mediated cyclization

โœ Scribed by Cy M. Jeffries; Stephen C. Graham; Philippa H. Stokes; Charles A. Collyer; J. Mitchell Guss; Jacqueline M. Matthews


Book ID
111752809
Publisher
Cold Spring Harbor Laboratory Press
Year
2006
Tongue
English
Weight
425 KB
Volume
15
Category
Article
ISSN
0961-8368

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Protein splicing in trans results in the ligation of two protein or peptide segments linked to appropriate intein fragments. We have characterized the trans-splicing reaction mediated by a naturally expressed, approximately 100-residue N-terminal fragment of the Mycobacterium tuberculosis intein and