𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Stability of hepatitis C virus RNA in blood samples by TaqMan real-time PCR

✍ Scribed by Kenan Sener; Mehmet Yapar; Orhan Bedir; Cem Gül; Ömer Coskun; Ayhan Kubar

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
112 KB
Volume
24
Category
Article
ISSN
0887-8013

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The storage conditions of blood samples for reliable results are very important in hepatitis C virus (HCV) RNA amplification tests used in routine HCV analyses. According to many studies, storage conditions could affect the RNA stability for HCV RNA detection. We have studied HCV RNA stability in blood samples stored at 4°C. Nineteen blood samples containing different HCV RNA levels were stored at 4°C and they were then analyzed by TaqMAN real‐time PCR method. HCV RNA levels remained almost stable (100%) at least for five weeks at this storage condition. However, among them, the stability period was up to 11 weeks in two of the samples. As with these findings, there was a slightly significant correlation between the positivity time and the beginning HCV RNA levels (r=0.474, P=0.040). We conclude that, blood samples can be stored at 4°C for five weeks without any significant difference in detected HCV RNA level by using TaqMan real‐time PCR. J. Clin. Lab. Anal. 24:134–138, 2010. © 2010 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

The Cobas AmpliPrep-Cobas TaqMan real-ti
The Cobas AmpliPrep-Cobas TaqMan real-time polymerase chain reaction assay fails to detect hepatitis C virus RNA in highly viremic genotype 4 clinical samples
✍ Stéphane Chevaliez; Magali Bouvier-Alias; Laurent Castéra; Jean-Michel Pawlotsky 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB 👁 2 views

LT). Six patients (30%) with LF were not treated medically, of whom five had submassive necrosis on liver biopsy. Five of these six patients underwent transplantation and all but one survived. One could not undergo transplantation due to development of fatal cholangitis. In conclusion, about 20% of

Detection of human bocavirus in respirat
Detection of human bocavirus in respiratory, fecal, and blood samples by real-time PCR
✍ Sarah J. Tozer; Stephen B. Lambert; David M. Whiley; Seweryn Bialasiewicz; Micha 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 79 KB

## Abstract Human bocavirus (HBoV) has been detected worldwide in respiratory samples. Two real‐time PCR assays, targeting the non‐structural protein (NP‐1) and viral protein (VP‐1) genes, were designed and validated to detect HBoV in patients with respiratory disease, gastroenteritis, or systemic

Validation of single real-time TaqMan® P
Validation of single real-time TaqMan® PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens
✍ V. Enouf; G. Dos Reis; J.P. Guthmann; P.J. Guerin; M. Caron; V. Marechal; E. Nic 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 205 KB 👁 1 views

Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt