𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Validation of single real-time TaqMan® PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens

✍ Scribed by V. Enouf; G. Dos Reis; J.P. Guthmann; P.J. Guerin; M. Caron; V. Marechal; E. Nicand

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
205 KB
Volume
78
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt 5207-5292) for the detection and quantification of the four major genotypes of HEV. This assay proved to be as efficient as the conventional RT-PCR methodology for the detection of HEV in clinical samples testing positive previously. The real-time RT-PCR and conventional RT-PCR were performed comparatively on 60 pairs of sera and stools collected during a recent outbreak of hepatitis E in Darfur. The real-time RT-PCR assay was 10- to 100-fold sensitive than for conventional RT-PCR assays used in this study with a range quantitation from 1.8 x 10(1) to 7.2 x 10(3) RNA copies/microl in clinical samples (serum and stools).

📜 SIMILAR VOLUMES

Comparison of real-time fluorescent RT-P
Comparison of real-time fluorescent RT-PCR and conventional RT-PCR for the detection of hepatitis E virus genotypes prevalent in China
✍ Chenyan Zhao; Zhuo Li; Baoshan Yan; Tim J. Harrison; Xinhui Guo; Feng Zhang; Jim 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 1 views

## Abstract To compare the specificity and sensitivity of a real‐time fluorescent RT‐PCR assay with conventional RT‐PCR, sera from 110 healthy blood donors, 120 patients with a clinical diagnosis of chronic hepatitis B, and 416 patients with non‐A‐C acute hepatitis, as well as serial dilutions of H

Validation of a sensitive and specific r
Validation of a sensitive and specific real-time PCR for detection and quantitation of hepatitis B virus covalently closed circular DNA in plasma of chronic hepatitis B patients
✍ R.B. Takkenberg; H.L. Zaaijer; R. Molenkamp; S. Menting; V. Terpstra; C.J. Weegi 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 128 KB 👁 1 views

## Abstract Hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) serves as a template for viral replication and plays a role in persistence of HBV infection. The origin and significance of cccDNA in plasma however, is not well understood. A sensitive, specific, and reproducible real‐time

Rapid quantitative PCR assays for the si
Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens
✍ I. Engelmann; D.R. Petzold; A. Kosinska; B.G. Hepkema; T.F. Schulz; A. Heim 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB 👁 1 views

## Abstract Rapid diagnosis of human herpesvirus primary infections or reactivations is facilitated by quantitative PCRs. Quantitative PCR assays with a standard thermal cycling profile permitting simultaneous detection of herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (C

A real-time PCR assay to identify and di
A real-time PCR assay to identify and discriminate among wild-type and vaccine strains of varicella-zoster virus and herpes simplex virus in clinical specimens, and comparison with the clinical diagnoses
✍ Ruth Harbecke; Michael N. Oxman; Beth A. Arnold; Charlotte Ip; Gary R. Johnson; 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 251 KB

## Abstract A real‐time PCR assay was developed to identify varicella‐zoster virus (VZV) and herpes simplex virus (HSV) DNA in clinical specimens from subjects with suspected herpes zoster (HZ; shingles). Three sets of primers and probes were used in separate PCR reactions to detect and discriminat