Cytochrome P-450 in whole liver homogenates, which contain an appreciable amount of hemoglobin, is detected by dithionite-difference spectroscopy of CO-bubbled homogenates. The molar extinction difference of cytocrhome P-450 by this method was determined to be 104 mM-'cm-r by comparative observation
Stability of cytochrome P450 proteins in cultured precision-cut rat liver slices
β Scribed by Elham Hashemi; Clive Till; Costas Ioannides
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 374 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0300-483X
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The cytochrome P-450 content promptly fell in adult rat hepatocytes cultured in conventional conditions whereas no obvious change was detected over a 10-day period when these cells were co-cultured with another rat liver epithelial cell type. A concomitant maintenance of the amino-pyrine N-demethyla