Quantitative determination of cytochrome P-450 in rat liver homogenate

✍ Scribed by Takashi Matsubara; Masahiro Koike; Akira Touchi; Yoshihiro Tochino; Koichi Sugeno

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 530 KB
• Volume: 75
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90114-7

✦ Synopsis

Cytochrome P-450 in whole liver homogenates, which contain an appreciable amount of hemoglobin, is detected by dithionite-difference spectroscopy of CO-bubbled homogenates. The molar extinction difference of cytocrhome P-450 by this method was determined to be 104 mM-'cm-r by comparative observations of the absorbance change in the dithionite-and CO-difference spectra of the membrane-bound hemoprotein. The content of cytochrome P-450 in normal rat liver was estimated to be 50 nmolig wet weight of liver, and increased significantly after pretreatment of the animals with either phenobarbital or 3-methylcholanthrene.

Methods

Wistar-strain male rats, weighing 250-300 g, were used for the experiments. Some of the animals received daily intraperitoneal injections of 596 Copynght