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Stability of a highly charged noncovalent complex in the gas phase: holomyoglobin

โœ Scribed by Yu-Luan Chen; J. M. Campbell; B. A. Collings; Lars Konermann; D. J. Douglas

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
177 KB
Volume
12
Category
Article
ISSN
0951-4198

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โœฆ Synopsis

Gas phase holomyoglobin (hMb) ions in charge states 7 to 21 were formed by electrospray ionization in combination with a continuous-flow mixing apparatus. Collision cross section measurements show that the highly charged ions are somewhat unfolded in comparison to low charge states but still retain a considerable degree of folding. A new collision model is presented which calculates the relative energies transferred to complexes in tandem mass spectrometry. Tandem mass spectrometry and ion trapping experiments both show that the energies required to dissociate heme from the highly charged heme-protein complexes in the gas phase are similar to those of low charge states, previously shown in literature ion cyclotron resonance experiments to be 0.7-1.0 eV. These energies are comparable to those of the heme binding energy in solution. The results suggest that even for the highly charged hMb ions which have unfolded somewhat, the heme-protein interactions remain relatively unperturbed.

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