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Stability and activity of a thermostable malic enzyme in denaturants and water-miscible organic solvents

✍ Scribed by Annamaria GUAGLIARDI; Giuseppe MANCO; Mossé ROSSI; Simonetta BARTOLUCCI


Book ID
115126376
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
676 KB
Volume
183
Category
Article
ISSN
1432-1327

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On enzymatic activity in organic solvent
✍ Tao Ke; Alexander M. Klibanov 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 63 KB 👁 2 views

Catalytic activities of ␣-chymotrypsin and subtilisin Carlsberg in various hydrous organic solvents were measured as a function of how the enzyme suspension had been prepared. In one method, lyophilized enzyme was directly suspended in the solvent containing 1% water. In another, the enzyme was prec