Influence of Water Miscible Organic Solvents on α-chymotrypsin in Solution and Immobilized on Eupergit CM
✍ Scribed by Linus Olofsson; Pernilla Söderberg; Ian A. Nicholls
- Book ID
- 106363540
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 202 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
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Immobilized a-chymotrypsin was used as catalyst to synthesize a kyotorphin derivative (Bz-Tyr-Arg-OEt) in the presence of five water-miscible aprotic solvents (dimethylsulphoxide, dimethylformamide, acetonitrile, acetone and tetrahydrofurane) at 30 °C. By using a kineticallycontrolled approach, the
Two different immobilized chymotrypsin derivatives were used to synthesize kyotorphin, using Nbenzoyl-L-tyrosine ethyl ester and L-arginine ethyl ester as substrates, in water-DMF media. The first was adsorbed onto Celite particles and the second was multipoint covalently attached into polyacrylamid