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Stabilities and Conformational Transitions of Various Proteases in the Presence of an Organic Solvent

✍ Scribed by Hiroyasu Ogino; Yuichi Gemba; Yoshikazu Yutori; Noriyuki Doukyu; Kosaku Ishimi; Haruo Ishikawa

Book ID
109387509
Publisher
American Institute of Chemical Engineers
Year
2007
Tongue
English
Weight
250 KB
Volume
23
Category
Article
ISSN
8756-7938

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## Abstract Engineered extremely thermostable variants of the thermolysin‐like protease from __Bacillus stearothermophilus__ possessing an introduced disulfide bond G8C/N60C (double mutant, DM) and six additional amino acid substitutions in the exposed loop region 56–69 (Boilysin, BLN) have been pr