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Spotlights on our sister journals: Eur. J. Inorg. Chem. 7/2009

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
601 KB
Volume
2009
Category
Article
ISSN
1434-1948

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โœฆ Synopsis

Enzymatic Incorporation of Emissive Pyrimidine Ribonucleotides

Making an emission: T7 RNA polymerase incorporates a series of thiophene-modified uridine triphosphate (UTP) analogues to generate emissive RNA transcripts. Labeling experiments suggested that the enzyme frequently pauses at the incorporation position and, when incorporation does take place, T7 RNA polymerase fails to elongate the modified oligonucleotides and yields aborted transcripts. TPs = triphosphates.

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