Spontaneous temperature-sensitive mutants ofPseudomonas cepacia: Isolation and utilization
β Scribed by Anne Morris Hooke; Renee C. Palecek; Theresa M. Palecek; Deidre R. MIller
- Publisher
- Springer
- Year
- 1991
- Tongue
- English
- Weight
- 374 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0343-8651
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A procedure for the isolation of spontaneous temperature sensitive mutants of Escherichia coli has been developed. They are selected as survivors at high temperature against the combined killing effects exerted by a temperature inducible lambda prophage and either streptomycin plus ampillicin or amp
Mutants temperature-sensitive for growth have been isolated from the established line of Chinese hamster fibroblasts Wg1A. These mutants, together with the ones previously isolated by Roscoe et al. ('73), have been characterized with regard to their cell cycle properties. Most of them become arreste