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Spontaneous morphological changes by overexpression of the growth-associated protein B-50/GAP-43 in a PC12 cell line

โœ Scribed by Nielander, H.B.; French, P.; Oestreicher, A.B.; Gispen, W.H.; Schotman, P.

Book ID
121205169
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
639 KB
Volume
162
Category
Article
ISSN
0304-3940

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Quantitation of the growth-associated pr
Quantitation of the growth-associated protein B-50/GAP-43 and neurite outgrowth in PC12 cells
โœ E. R. A. Jap Tjoen San; M. H. Schmidt-Michels; B. M. Spruijt; A. B. Oestreicher; ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 546 KB

A combined assay to measure neurite outgrowth and B-50/GAP-43 levels in PC12 cells is reported. During NGF-induced neuritogenesis, B-50/GAP-43 expression was monitored by enzyme-linked immunosorbent assay (ELISA). Neurite outgrowth was quantified at the same time by the use of video image analysis.