Spontaneous decomposition of glutamine in cell culture media
โ Scribed by G.L. Tritsch; G.E. Moore
- Book ID
- 118874403
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 303 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0014-4827
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Glutamine is an essential nutrient in insect cell culture and plays a role in maximizing protein expression in baculovirus-infected cells. This paper presents a technique which combines two enzymatic reactions, specifically the deamination of glutamine via glutaminase and the subsequent amination of
The purpose of this study was to develop noninvasive techniques to monitor the composition of cell culture media in insect cell bioreactors. Such a monitor could be used in conjunction with a fed-batch feeding scheme to ensure that cells are maintained in an optimal environment for growth and protei
We present the setup of a flow injection analysis system designed for on-line monitoring of glutamate and glutamine. These amino acids represent a major energy source in mammalian cell culture. A cycling assay consisting of glutamate dehydrogenase and aspartate aminotransferase produces NADH proport