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Glutamine determination in insect cell culture media

✍ Scribed by Min Ying Wang; Kelly Wester; William E. Bentley

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 356 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00156359

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✦ Synopsis

Glutamine is an essential nutrient in insect cell culture and plays a role in maximizing protein expression in baculovirus-infected cells. This paper presents a technique which combines two enzymatic reactions, specifically the deamination of glutamine via glutaminase and the subsequent amination of 2-oxoglutarate to glutamate, to measure the concentration of glutamine in insect cell media. A specific ghttamine uptake rate of 4.2x lo-* umol/lO%ells/hr was determined for infected cells by this method.

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