Splitless injection of up to hundreds of microliters of liquid samples in capillary GC: Part II, experimental results

✍ Scribed by Grob, Konrad ;Brem, Sandra

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 837 KB
• Volume: 15
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240151105

✦ Synopsis

Abstract

Sample evaporation in splitless injection of large volumes is rapid: depending on the experiment, results indicate that 200 μl of hexane, for instance, evaporates in 2–10 s, producing vapor at a rate of many hundreds of milliliters per minute. A 60 × 4 mm packed bed of 20–35 mesh Tenax TA enabled injection of 200 μl volumes of all solvents tested, and even 1 ml injections were possible provided they were performed over a period of 30 s. Retention of volatile sample components depends on the sample solvent, the injection volume, and the injection speed, but only little on the injector temperature. Losses of n‐tridecane varied from hardly 15 % (when dissolved in pentane) to ca 60 % (ethyl acetate); losses of n‐heptadecane were usually below 20 %. The column temperature during injection should be at least ca 20–30°C above the standard solvent boiling point.