Spindle disturbances in human–hamster hybrid (AL) cells induced by the electrical component of the mobile communication frequency range signal

✍ Scribed by Thorsten Schrader; Thomas Kleine-Ostmann; Klaus Münter; Christian Jastrow; Ernst Schmid

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 139 KB
• Volume: 32
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/bem.20634

✦ Synopsis

The production of spindle disturbances in a human-hamster hybrid (A L ) cell line by an electromagnetic field (EMF) with field strength of 90 V/m at a frequency of 900 MHz was studied in greater detail. The experimental setup presented allows investigating whether either the electrical (E) and/or the magnetic (H) field component of EMF can be associated with the effectiveness of the spindledisturbing potential. Therefore, both field components of a transversal electromagnetic field (TEM) wave have been separated during exposure of the biological system. This procedure should give more insight on understanding the underlying mechanisms of non-thermal effects of EMF. A statistical comparison of the proportions of the fractions of ana-and telophases with spindle disturbances, obtained for five different exposure conditions with respect to unexposed controls (sham condition), showed that only cells exposed to the H-field component of the EMF were not different from the control. Therefore, the results of the present study indicate that an exposure of cells to EMF at E-field strengths of 45 and 90 V/m, as well as to the separated E component of the EMF, induces significant spindle disturbances in ana-and telophases of the cell cycle. Bioelectromagnetics 32:291-301, 2011.