Spindle disturbances in human-hamster hybrid (AL) cells induced by mobile communication frequency range signals

✍ Scribed by Thorsten Schrader; Klaus Münter; Thomas Kleine-Ostmann; Ernst Schmid

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 232 KB
• Volume: 29
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/bem.20428

✦ Synopsis

Abstract

The production of spindle disturbances in FC2 cells, a human‐hamster hybrid (A~L~) cell line, by non‐ionizing radiation was studied using an electromagnetic field with a field strength of 90 V/m at a frequency of 835 MHz. Due to the given experimental conditions slide flask cultures were exposed at room temperature in a µTEM (transversal electromagnetic field) cell, which allows optimal experimental conditions for small samples of biological material. Numerical calculations suggest that specific absorption rates of up to 60 mW/kg are reached for maximum field exposure. All exposure field parameters—either measured or calculable—are precisely defined and, for the first time, traceable to the standards of the SI system of physical units. Compared with co‐incident negative controls, the results of two independently performed experiments suggest that exposure periods of time from 0.5 to 2 h with an electric field strength of 90 V/m are spindle acting agents as predominately indicated by the appearance of spindle disturbances at the ana‐ and telophase stages (especially lagging and non‐disjunction of single chromosomes) of cell divisions. The spindle disturbances do not change the fraction of mitotic cells with increasing exposure time up to 2 h. Due to the applied experimental conditions an influence of temperature as a confounder parameter for spindle disturbances can be excluded. Bioelectromagnetics 29:626–639, 2008. © 2008 Wiley‐Liss, Inc.