Spin-label assay for lysozyme
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Laurance S. Johnston; Francis C. Neuhaus
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Article
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1978
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Elsevier Science
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English
β 393 KB
A spin-label assay for lysozyme, which is based on the enzymatic hydrolysis of spin-labeled peptidoglycan, is described. Hydrolysis of this polymer by lysozyme results in sharpening of the esr spectrum. The rate of spectral sharpening is a function of enzyme concentration. When the activities of hen