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Sperm status and DNA dose play key roles in sperm/ICSI-mediated gene transfer in caprine

โœ Scribed by Farshad Shadanloo; Mohammad Hasan Najafi; Sayed Morteza Hosseini; Mehdi Hajian; Mohsen Forouzanfar; Kamran Ghaedi; Parvaneh Abedi; Somaye Ostadhosseini; Laleh Hosseini; Morad-Pasha Eskandari-Nasab; Mohammad Hossein Nasr Esfahani

Book ID
111664762
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
178 KB
Volume
77
Category
Article
ISSN
1040-452X

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We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin