𝔖 Bobbio Scriptorium
✦   LIBER   ✦

DNA dose and sequence dependence in sperm-mediated gene transfer

✍ Scribed by Ilaria Sciamanna; Simona Piccoli; Laura Barberi; Germana Zaccagnini; Anna Rosa Magnano; Roberto Giordano; Paolo Campedelli; Clague Hodgson; Rodolfo Lorenzini; Corrado Spadafora

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
112 KB
Volume
56
Category
Article
ISSN
1040-452X

No coin nor oath required. For personal study only.

✦ Synopsis

We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive embryo lethality and yet increases the yield of genetic transformation among born animals of both species compared to viral constructs. Arrest of embryonic development is a DNA dose-dependent effect, which is observed with high DNA doses, while lower doses are compatible with development. Finally, the overall efficiency of sperm-mediated gene transfer is higher when ejaculated, versus epididymal, spermatozoa are used. We suggest that this difference is related to the highly efficient apoptotic response in epididymal compared to ejaculated spermatozoa, triggered by the interaction of exogenous DNA molecules with the sperm membrane.

πŸ“œ SIMILAR VOLUMES

Sperm-mediated gene transfer studies on
Sperm-mediated gene transfer studies on zebrafish in Singapore
✍ Hong-Woo Khoo πŸ“‚ Article πŸ“… 2000 πŸ› John Wiley and Sons 🌐 English βš– 56 KB

This paper describes the work conducted in the Department of Biological Sciences, National University of Singapore; on sperm-mediated gene transfer in zebrafish. This study began by carrying out direct interaction experiments of pUSVCAT reporter DNA with spermatozoa. Other constructs, including pXGH

Sperm-mediated gene transfer in the silk
Sperm-mediated gene transfer in the silkworm Bombyx mori
✍ Yusuff Shamila; Sinnakaruppan Mathavan πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 251 KB

Plasmid DNA containing the CAT reporter gene was injected into the testis of V instar silkworm larvae. The persistence, expression, and transmission of the injected DNA were monitored in the injected individuals till eclosion as well as in the progeny. The DNA injected into the testis persisted extr

Sequence-specific gene silencing in muri
Sequence-specific gene silencing in murine muscle induced by electroporation-mediated transfer of short interfering RNA
✍ Tsunao Kishida; Hidetsugu Asada; Satoshi Gojo; Suzuyo Ohashi; Masaharu Shin-Ya; πŸ“‚ Article πŸ“… 2004 πŸ› John Wiley and Sons 🌐 English βš– 156 KB

## Abstract ## Background Post‐genomic biomedical research requires efficient techniques for functional analyses of poorly characterized genes in living organisms. Sequence‐specific gene silencing in mammalian organs may provide valuable information on the physiological and pathological roles of p

Improving plasmid DNA-mediated liver gen
Improving plasmid DNA-mediated liver gene transfer by prolonging its retention in the hepatic vasculature
✍ Feng Liu; Leaf Huang πŸ“‚ Article πŸ“… 2001 πŸ› John Wiley and Sons 🌐 English βš– 196 KB

Background Naked DNA is the simplest and safest method to deliver genes to the liver. In this study, we demonstrate that significant gene expression could be achieved in the liver by transiently restricting blood flow through the liver immediately following peripheral intravenous injection of plasmi

Recovery of hormonal regulation in prote
Recovery of hormonal regulation in protein kinase defective adrenal cells through DNA-mediated gene transfer
✍ Bernard P. Schimmer; Margaret Wong; Donalee O'Brien; Patricia Schulz πŸ“‚ Article πŸ“… 1986 πŸ› John Wiley and Sons 🌐 English βš– 840 KB

A CAMP-resistant mutant (Kin-8) isolated from Y1 mouse adrenocortical tumor cells harbors a specific lesion in the regulatory subunit of the type 1 CAMPdependent protein kinase. This mutant also is resistant to the effects of corticotropin and cAMP on steroidogenesis, growth and morphology, suggesti