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Sperm-mediated gene transfer in the silkworm Bombyx mori

โœ Scribed by Yusuff Shamila; Sinnakaruppan Mathavan

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
251 KB
Volume
37
Category
Article
ISSN
0739-4462

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โœฆ Synopsis

Plasmid DNA containing the CAT reporter gene was injected into the testis of V instar silkworm larvae. The persistence, expression, and transmission of the injected DNA were monitored in the injected individuals till eclosion as well as in the progeny. The DNA injected into the testis persisted extrachromosomally during the entire period of metamorphosis and was also transferred into the egg via sperm during fertilization. Injected plasmids were rescued from the moths that emerged from the injected larvae and also from the eggs laid by the moths that copulated with injected males. Positive signals for CAT assay in the experimental samples suggested that the injected DNA was internalized in the testicular cells and sperm. The persistence, expression, and transmission of the DNA injected into the testes indicate that sperm-mediated gene transfer is possible in the silkworm,

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Sperm/DNA interaction: DNA binding proteins in sperm cell of silkworm Bombyx mori
โœ Y. Shamila; S. Mathavan ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 117 KB ๐Ÿ‘ 2 views

Plasmid DNA transferred into testes of silkworm larvae following in vivo gene transfer method was taken up by the sperm cells during spermatogenesis and the DNA was transferred to Go and subsequent progeny. RT-PCR analysis confirmed the internalization of the DNA taken up by the sperm. Specific clas