Spectroscopic study of permanganate oxidation reactions of oligonucleotides containing single base mismatches

✍ Scribed by Chinh T. Bui; Andreana Lambrinakos; Richard G. H. Cotton

• Publisher: Wiley (John Wiley & Sons)
• Year: 2003
• Tongue: English
• Weight: 201 KB
• Volume: 70
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.10543

✦ Synopsis

Abstract

The electrophoretic gel‐based chemical cleavage of the mismatch method gives an incomplete view of the DNA conformational changes induced by a single base mismatch. This spectroscopic study investigates the permanganate oxidation reactions with matched and mismatched DNA under constant and variable temperature conditions. The results, which include the oxidation levels, reaction patterns with isosbestic points, color changes, thermal spectra, spectroscopy derivative, and gel separation and melting temperatures, provide a fundamental background for identification of oligonucleotides containing single base mismatches by chemical means. © 2003 Wiley Periodicals, Inc. Biopolymers (Biospectroscopy) 70: 628–636, 2003