Spectroscopic Determination of Lysozyme Conformational Changes in the Presence of Trehalose and Guanidine
✍ Scribed by Barreca, Davide; Laganà , Giuseppina; Ficarra, Silvana; Gattuso, Giuseppe; Magazù, Salvatore; Torre, Roberto; Tellone, Ester; Bellocco, Ersilia
- Book ID
- 120447482
- Publisher
- Springer-Verlag
- Year
- 2012
- Tongue
- English
- Weight
- 692 KB
- Volume
- 66
- Category
- Article
- ISSN
- 1085-9195
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Infrared and Fourier transform Raman spectra are reported for dried mixtures of trehalose and lysozyme. The Raman spectra show effects on the protein amide I band and some sugar hands that are not present when the components are dried separately. Comparison of ir spectra with those published previou
In the present study, the thermal unfolding of lysozyme in D 2 O and D 2 O/CH 3 CH 2 OD solutions is analyzed by means of FTIR spectroscopy. A detailed investigation of the conformational rearrangement of the protein upon heating is achieved by inspection of the amide bands. The effects of partial a