A simple and reliable method is described for the determination of glycosaminoglycans and proteoglycans in tissue extracts as well as during preparative and analytical procedures for these molecules. It is particularly useful because it requires much less starting material for the identification of
Spectrophotometric Method for the Assay of Glycosaminoglycans and Glycosaminoglycan-Depolymerizing Enzymes
β Scribed by K.A. Homer; L. Denbow; D. Beighton
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 582 KB
- Volume
- 214
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
A simple and rapid homogeneous enzyme-based binding assay is described to study the degree of interaction between glycosaminoglycans and various macromolecules/peptides. The method is based on the homogeneous inhibition of a highly positively charged enzyme, acid deoxyribonuclease II (EC 3.1.22.1),
A purpose-built fluorimeter for the assay of glycosaminoglycans has been constructed and extensively used. The technique uses the fluorescence of the cationic dye a&dine orange and the changes that accompany its interaction with the anionic sites of glycosaminoglycans. The operator titrates a volume