A simple purpose-built fluorimeter for the titrimetric assay of glycosaminoglycans

✍ Scribed by Gregory P. Diakun; Haydn E. Edwards; John C. Allen; Glyn O. Phillips; Robert B. Cundall

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 385 KB
• Volume: 94
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90376-2

✦ Synopsis

A purpose-built fluorimeter for the assay of glycosaminoglycans has been constructed and extensively used. The technique uses the fluorescence of the cationic dye a&dine orange and the changes that accompany its interaction with the anionic sites of glycosaminoglycans. The operator titrates a volume .of glycosaminoglycan solution from a burette into the cell containing acridine orange and the fluorescent changes are recorded on a digital multimeter. The data obtained are plotted and the equivalence point of the titration is calculated. In minutes, information can be obtained about the concentration of glycosaminoglycans, the degree of ionization of the anionic sites, the availability of anionic sites in glycosaminoglycan-protein mixtures, and the strength of the dye-polyanion interaction. This fluorimeter will prove to be a valuable addition to biochemical, medical, and food science laboratories.