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Spectrophotometric measurement of pH gradients in continuous-flow systems

✍ Scribed by S. Sagrado Vives; M.J. Medina Hernández; J.L. Martín Herrera; G. Ramis Ramos

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
908 KB
Volume
268
Category
Article
ISSN
0003-2670

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✦ Synopsis

In the presence of an acid-base mdlcator and usmg fast diode-array spectrophotometrlc scans, pH gradients m the spectrophotometnc cell can be measured at the same time as any other colonmetnc flow-mjectlon (FI) determmatlon 1s performed The effects of refractwe index changes, adsorptlon-desorptlon processes at the tube walls and assoclatlon of the mdlcator species with the system to be Investigated are considered Effectlve correction for the first two effects 1s demonstrated Indicators with different molecular structures and polantles gave slrmlar pH gradients, suggestmg adequate accuracy for most potential apphcatlons Apphcatlon to the detection and correction of systematic and random errors m pH-sensitive FI procedures IS demonstrated using the o-phthalaldehyde-iv-acetyl-L-cysteme method for bovme serum albumin

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